牛牛游戏厅安卓,{原始关键词}

    <dir id='soubn'><del id='soubn'><del id='soubn'></del><pre id='soubn'><pre id='soubn'><option id='soubn'><address id='soubn'></address><bdo id='soubn'><tr id='soubn'><acronym id='soubn'><pre id='soubn'></pre></acronym><div id='soubn'></div></tr></bdo></option></pre><small id='soubn'><address id='soubn'><u id='soubn'><legend id='soubn'><option id='soubn'><abbr id='soubn'></abbr><li id='soubn'><pre id='soubn'></pre></li></option></legend><select id='soubn'></select></u></address></small></pre></del><sup id='soubn'></sup><blockquote id='soubn'><dt id='soubn'></dt></blockquote><blockquote id='soubn'></blockquote></dir><tt id='soubn'></tt><u id='soubn'><tt id='soubn'><form id='soubn'></form></tt><td id='soubn'><dt id='soubn'></dt></td></u>
  1. <code id='soubn'><i id='soubn'><q id='soubn'><legend id='soubn'><pre id='soubn'><style id='soubn'><acronym id='soubn'><i id='soubn'><form id='soubn'><option id='soubn'><center id='soubn'></center></option></form></i></acronym></style><tt id='soubn'></tt></pre></legend></q></i></code><center id='soubn'></center>

      <dd id='soubn'></dd>

        <style id='soubn'></style><sub id='soubn'><dfn id='soubn'><abbr id='soubn'><big id='soubn'><bdo id='soubn'></bdo></big></abbr></dfn></sub>
        <dir id='soubn'></dir>
      1. <tr id='0h5h2'><strong id='0h5h2'></strong><small id='0h5h2'></small><button id='0h5h2'></button><li id='0h5h2'><noscript id='0h5h2'><big id='0h5h2'></big><dt id='0h5h2'></dt></noscript></li></tr><ol id='0h5h2'><option id='0h5h2'><table id='0h5h2'><blockquote id='0h5h2'><tbody id='0h5h2'></tbody></blockquote></table></option></ol><u id='0h5h2'></u><kbd id='0h5h2'><kbd id='0h5h2'></kbd></kbd>

        <code id='0h5h2'><strong id='0h5h2'></strong></code>

        <fieldset id='0h5h2'></fieldset>
              <span id='0h5h2'></span>

                  <ins id='0h5h2'></ins>
                  <acronym id='0h5h2'><em id='0h5h2'></em><td id='0h5h2'><div id='0h5h2'></div></td></acronym><address id='0h5h2'><big id='0h5h2'><big id='0h5h2'></big><legend id='0h5h2'></legend></big></address>

                  <i id='0h5h2'><div id='0h5h2'><ins id='0h5h2'></ins></div></i>
                  <i id='0h5h2'></i>
                1. <dl id='0h5h2'></dl>
                  1. 当前位置: 代写一篇论文多少钱 > 医学论文 > 手足口病患者样本特异性核酸检测分析

                    手足口病患者样本特异性核酸检测分析

                    时间:2020-02-13 11:23作者:仲惟惟 尹晔
                    本文导读:这是一篇关于手足口病患者样本特异性核酸检测分析的文章,手足口病是由多种人肠道病毒引起的一种儿童常见传染病,大多数患者症状轻微,以发热和手、足、口腔等部位的皮疹或疱疹为主要症状。

                      摘    要: 目的 比较手足口病患者咽拭、粪便样本中肠道病毒(EV)的检出率,为手足口病监测样本采集的选择提供依据。方法 采集2013年间鞍山地区医疗机构临床诊断手足口病患者63例咽拭、粪便配对标本。采用Real-time PCR法进行柯萨奇病毒A组16型(Cox A16)、肠道病毒71型(EV 71)、其他肠道病毒型检测。结果 63例患者配对样本中,Cox A16、EV 71、肠道病毒通用型咽拭子阳性率分别为26.98%、1.59%和26.98%,粪便阳性率分别为22.22%、1.59%和31.75%。结果显示两种样本病毒核酸阳性率,差异无统计学意义(χ2=0.534,P>0.05)。两种样本检测结果相关性,差异有统计学意义(χ2=8.036,P<0.05)。咽拭样本和粪便配对样本核酸检测阳性率,差异无统计学意义(P>0.05)。结论 咽拭样本和粪便样本核酸检测阳性率相同,可以采集咽拭样本代替粪便标本。

                      关键词:牛牛游戏厅安卓,{原始关键词} 手足口病; 病原学; 肠道病毒;

                      Abstract: Objective To compare the detection rate of enterovirus(EV)in swallow swab and fecal samples of patients with hand-foot-mouth disease, and to provide evidence for the monitoring sample selection of hand-foot-mouth disease. Method In 2013, 63 patients with hand-foot-mouth disease clinically diagnosed by medical institutions in Anshan district were collected and paired samples of pharyngeal swabs and feces were collected.Cox A16, EV 71 and other enter virus types were detected by real-time PCR. Results Among the 63 pairs of specimens, the positive rates of Cox A16,EV 71 and other Etiology in throat swabs were 26.98%, 1.59% and 31.75% respectively, and in excrements were 22.22%, 1.59% and 31.75%respectively. The results showed that the positive rate of viral nucleic acid in the two samples was not statistically significant(χ2=0.534,P>0.05). The correlation between the two samples was statistically significant(χ2=8.036,P<0.05). There was no significant difference in the positive rate of nucleic acid detection between the throat swab and the fecal paired sample(P>0.05). Conclusion The positive rate of nucleic acid detection in throat swab and fecal samples is the same, and throat swab samples can be taken instead of fecal specimens.

                      Keyword: Hand-foot-mouth disease; Etiology; Enterovirus;

                    牛牛游戏厅安卓,{原始关键词}   SHOUZUKOUBINGSHIYOUDUOZHONGRENCHANGDAOBINGDUYINQIDEYIZHONGERTONGCHANGJIANCHUANRANBING,DADUOSHUHUANZHEZHENGZHUANGQINGWEI,YIFAREHESHOU、ZU、KOUQIANGDENGBUWEIDEPIZHENHUOPAOZHENWEIZHUYAOZHENGZHUANG。SHAOSHUHUANZHEKECHUXIANWUJUNXINGNAOMOYAN、NAOYAN、JIXINGCHIHUANXINGMABI、SHENJINGYUANXINGFEISHUIZHONGHEXINJIYANDENG,GEBIEZHONGZHENGHUANERBINGQINGJINZHANKUAI,KEDAOZHISIWANG。BENWENTONGGUOReal-time PCRFADUI2013NIANANSHANDIQU63LISHOUZUKOUBINGHUANZHEDEYANSHIHEFENBIANYANGBENJINXINGTEYIXINGHESUANJIANCEFENXI,WEISHOUZUKOUBINGJUJIXINGYIQING、BAOFAYIQINGHECHANGGUIJIANCEYANGBENCAIJITIGONGKEXUEYIJU,XIANBAOGAORUXIA。

                      1 、材料与方法

                      1.1 、样本来源

                    牛牛游戏厅安卓,{原始关键词}   CAIJI2013NIANANSHANDIQUYILIAOJIGOULINCHUANGZHENDUANWEISHOUZUKOUBINGHUANZHEFABING7TIANNEIDEYANGBEN,CAIJIHOU4℃BAOCUN,SONGANSHANSHIJIKONGJIANCE,RUGUODANGTIANBUNENGJIANCE,SONG-70℃BAOCUNDAIJIAN。GONGSHOUJI63LIHUANZHEDEYANSHIZIHEFENBIANBIAOBEN。NIANLING4~6SUI,NAN28LI,NV35LI。

                      1.2、 试剂与仪器

                      CAIYONGSHANGHAIPULUOMAIGESHENGWUCHANPINYOUXIANGONGSIDEpromega EV 71、Cox A16、CHANGDAOTONGYONGBINGDUJIANCESHIJIHE,SHIYONGBIO-RAD IQ5 Real-time PCR PCRJIANCEYIQI。

                      1.3 、检测方法

                      依据《手足口病预防控制指南》及试剂盒说明书,应用Real-time PCR法对柯萨奇病毒A组16型(Cox A16)、肠道病毒71型(EV 71)、其他肠道病毒进行检测。
                     

                    手足口病患者样本特异性核酸检测分析
                     

                      1.4 、统计学处理

                    牛牛游戏厅安卓,{原始关键词}   CAIYONGSPSS 16.0RUANJIANJINXINGTONGJIFENXI,JINGχ2JIANYAN,YIP<0.05WEICHAYIJUYOUTONGJIXUEYIYI。

                      2、 结果

                      2.1、 两种样本检出率

                      63LIHUANZHEYANGBENZHONG,Cox A16、EV 71、CHANGDAOBINGDUTONGYONGXINGYANSHIZIYANGXINGLVFENBIEWEI26.98%(17/63)、1.59%(1/63)HE26.98%(17/63),FENBIANYANGXINGLVFENBIEWEI22.22%(14/63)、1.59%(1/63)HE31.75%(20/63)。YANSHIHEFENBIANYANGBENBINGDUHESUANYANGXINGJIANCHULV,CHAYIWUTONGJIXUEYIYI(χ2=0.534,P>0.05)。

                      2.2、 两种样本配对比较

                    牛牛游戏厅安卓,{原始关键词}   LIANGZHONGYANGBENHESUANJIANCEYANGXINGLVZHIJIANCUNZAIGUANLIAN,CHAYIYOUTONGJIXUEYIYI(χ2=8.036,P<0.05)。LIANGZHONGYANGBENHESUANJIANCEJIEGUOMcNemarJIANYAN,CHAYIWUTONGJIXUEYIYI(P>0.05)。JIANBIAO1。

                      表1 两种样本病毒核酸检测配对比较情况(例)
                    表1 两种样本病毒核酸检测配对比较情况(例)

                      3、 讨论

                      ZI2008NIANSHOUZUKOUBINGLIERUFADINGCHUANRANBINGYILAI,ZAIWOGUODUODISHOUZUKOUBINGDOUYOUBAOFALIUXING[1,2,3]。ZHENDUIJUJIHUOZHEBAOFAYIQING,JISHICAIJIJIANCEYANGBENWEIHESHIYIQINGTIGONGYOULIYIJU。BENCIYANJIUJIEGUOXIANSHI,LIANGZHONGYANGBENHESUANJIANCEYANGXINGLVZHIJIANCUNZAIGUANLIAN,CHAYIYOUTONGJIXUEYIYI(P<0.05)。JIFENBIANYANGBENHESUANJIANCESHIYANGXING,YANSHIYANGBENHESUANJIANCEYESHIYANGXING。LIANGZHONGYANGBENHESUANJIANCEJIEGUO,CHAYIWUTONGJIXUEYIYI(P>0.05)。ZHEIBIAOMING,FENBIANYANGBENHEYANSHIYANGBENZAIYONGReal-time PCRFAJINXINGTEYIXINGHESUANJIANCEFENXISHI,YANGXINGLVJIEJIN。ZAIFASHENGJUJIHUOBAOFAYIQINGSHI,WEIFANGZHIFENBIANYANGBENBUNENGJISHISHANGSONG,KEYICAIJIYANSHIYANGBENJINXINGHESUANJIANCE,YIJISHIHESHIYIQING。

                      BENCIYANGBENSHOUJIYIKAOZHUYUANLINCHUANGYISHENGHEJIKONGJIGOU,BUTONGCAIJIZHECAIJIYANGBENCUNZAICHAYI。PIANYUANDIQUHUANZHEYANGBENDANGTIANBUNENGJIANCE,JIANCEJIEGUOCUNZAICHAYI。JINSHOUJI1NIANYANGBENJINXINGFENXI,JIANYIZHANGQIJIANCEFENXI。

                      参考文献

                      [1] 罗平编.饮料资料、饮料分析与检验[M].北京:中国轻工业出版社,1992:311-313.
                      [2] 邰昌松,肖慧贤.用气相色谱-质谱联用技术分析钩吻碱中毒[J].中国卫生检验杂志,1999,9(1):20.
                    牛牛游戏厅安卓,{原始关键词}   [3]Liu J,Wangs,Liu H,et al. The monitoring biomarker for immune of lymphocytes in the eidedy[J]. Mech Ageing Dev,1997,94(2):178.

                    联系我们
                    • 写作QQ:
                    • 发表QQ:
                    • 服务电话:
                    • 售后电话:
                    • 邮箱:
                    范文范例

                    豪运棋牌送20金币-牛牛游戏厅安卓,{原始关键词} 欢乐斗牛棋牌下载 番茄棋牌---HOME_Welcome 明豪棋牌---HOME_Welcome 天棋棋牌---HOME_欢迎您